Lions scrumhalf Michael Bondesio and centre Lionel Mapoe have both been ruled out for six weeks.
Bondesio sustained a grade-two quad strain in the match against the Cheetahs on Saturday, while Mapoe strained his right hamstring.
Meanwhile, Anthony Volmink and Elton Jantjies, who are recovering from shoulder injuries, will be considered for the Golden Lions' Vodacom Cup warm-up game against the Pumas this weekend.
Photo: Muzi Ntombela/BackpagePix